Influence of uraemia on the determination of blood glycohaemoglobin by HPLC, electrophoresis and affinity chromatography in diabetic and non-diabetic patients.

Autor: Herruer MH; Department of Clinical Chemistry, Stichting Deventer Ziekenhuizen, Deventer, The Netherlands., van Kooten EA, Sluiter HE, Zuijderhoudt FM
Jazyk: angličtina
Zdroj: European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies [Eur J Clin Chem Clin Biochem] 1994 May; Vol. 32 (5), pp. 361-4.
DOI: 10.1515/cclm.1994.32.5.361
Abstrakt: Glycohaemoglobin in human blood can be determined by several methods. We investigated three methods used in daily practice, viz. affinity chromatography, HPLC and electrophoresis. We examined the influence of uraemia in diabetic and non-diabetic patients on the results obtained with HPLC and electrophoresis and compared these with the results obtained with affinity chromatography. Our current method (electrophoresis) proved to be very sensitive to uraemia giving falsely elevated levels of HbA1. HPLC was not disturbed by uraemia.
Databáze: MEDLINE