| Autor: |
Hardy PM; Cambridge Research Biochemicals, Northwich, Cheshire, UK., Holland D, Scott S, Garman AJ, Newton CR, McLean MJ |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleic acids research [Nucleic Acids Res] 1994 Aug 11; Vol. 22 (15), pp. 2998-3004. |
| DOI: |
10.1093/nar/22.15.2998 |
| Abstrakt: |
In order to increase the efficiency of use of automated DNA synthesizers (i.e. the number of oligomers prepared per day), we have devised and prepared novel phosphoramidite reagents that contain a linking group which, while stable under the normal synthesis conditions, is cleaved under basic conditions. When one of these linkers is introduced at the desired position in the synthesis of an oligonucleotide, subsequent detritylation enables the synthesis of a second oligonucleotides sequence upon the first. During deprotection of the oligonucleotide with ammonium hydroxide, the chain is cleaved at either side of the points of introduction of the novel reagent, generating two oligonucleotides free in solution. These reagents are of particular use in applications where oligomers are used in pairs (such as PCR, chemical synthesis of genes etc.) and means that an automated synthesis facility can be used more efficiently, without the need for operator intervention, after the working day is over. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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