| Autor: |
Anacker RL, Gerloff RK, Thomas LA, Mann RE, Bickel WD |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1975 Jun; Vol. 11 (6), pp. 1203-9. |
| DOI: |
10.1128/iai.11.6.1203-1209.1975 |
| Abstrakt: |
The properties of Rickettsia rickettsii purified from infected chicken yolk sacs or mouse L cell cultures by sucrose density gradient centrifugation in a zonal rotor were examined in various ways. Rickettsiae derived from both L cells and yolk sacs had similar compositions: about 12% nitrogen, 1.5% phosphorus, 5% carbohydrate, and 30% fatty acids. On a dry-weight basis, purified rickettsiae were at least 2,000 times as effective as a commercial spotted fever vaccine in protecting guinea pigs against infection with spotted fever rickettsiae and mice against death from toxin of R. rickettsii. Gradient-purified rickettsiae (0.6 mug) induced a serological response in guinea pigs of the same magnitude as that stimulated by 1,600 mug of the commercial vaccine. Gradient-purified rickettsiae had little group reactivity in complement fixation tests but became anti-complementary upon storage. Microagglutination and hemagglutination tests with the purified antigen gave promise of usefulness in diagnosis of spotted fever. These results suggest that zonal centrifugation may be a valuable procedure for the preparation of R. rickettsii vaccine and diagnostic reagent. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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