Autor: |
Nassif NT; School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia., Glenn WK, Mackinlay AG |
Jazyk: |
angličtina |
Zdroj: |
Journal of molecular evolution [J Mol Evol] 1994 Jul; Vol. 39 (1), pp. 47-55. |
DOI: |
10.1007/BF00178248 |
Abstrakt: |
Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the beta-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the beta-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a beta-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence. |
Databáze: |
MEDLINE |
Externí odkaz: |
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