The organization of the beta-globin gene of the bivalve mollusc Anadara trapezia and its evolutionary relationship to other invertebrate and vertebrate globin genes.

Autor: Nassif NT; School of Biochemistry and Molecular Genetics, University of New South Wales, Kensington, Australia., Glenn WK, Mackinlay AG
Jazyk: angličtina
Zdroj: Journal of molecular evolution [J Mol Evol] 1994 Jul; Vol. 39 (1), pp. 47-55.
DOI: 10.1007/BF00178248
Abstrakt: Three pairs of oligonucleotide primers based on partial DNA and amino acid sequences were used in a combination of PCR experiments to amplify the beta-globin gene of the bivalve mollusc Anadara trapezia. The sequence of 2,139 bp presented contains the whole of the beta-globin gene with the exception of the 5' flanking sequence. This gene possesses the three-exon-and-two-intron gene structure typical of vertebrate globin genes but the lengths of the introns (762 bp and 690 bp, respectively) are only approximately half the size of those present in a beta-variant gene previously characterized from this organism. The encoded amino acid sequence shows two changes when compared to the previously published amino acid sequence.
Databáze: MEDLINE