| Autor: |
Lambrechts AC; Department of Molecular Biology, Dr. Daniel den Hoed Cancer Center, Rotterdam, The Netherlands., Dorssers LC, Hupkes PE, van Gurp R, Baumgarten R, Hagemeijer A, van 't Veer MB, Touw IP |
| Jazyk: |
angličtina |
| Zdroj: |
Leukemia [Leukemia] 1994 Jul; Vol. 8 (7), pp. 1164-71. |
| Abstrakt: |
We generated a new lymphoma cell line carrying the translocations (8;14) and (14;18) and studied the genomic organization and expression of the BCL-2 and MYC genes. Polymerase chain reaction (PCR) and Southern analysis showed that the breakpoints of t(14;18) were located in the major breakpoint region (mbr) of the BCL-2 gene and just 5' of JH6 in the IgH locus. The breakpoints of the t(8;14) were located upstream of exon 2 in the non-coding region of the MYC gene and near the switch region of the IgH locus. Both IgH loci were involved in chromosomal translocations resulting in the absence of a functional B-cell receptor. Normal BCL-2 and truncated MYC transcripts were detected in these cells. The BCL-2 protein was expressed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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