| Autor: |
Ivanov KI; Department of Virology, Moscow State University, Russian Federation., Ivanov PA, Timofeeva EK, Dorokhov YL, Atabekov JG |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1994 Jun 13; Vol. 346 (2-3), pp. 217-20. |
| DOI: |
10.1016/0014-5793(94)00477-3 |
| Abstrakt: |
The movement proteins of two tobamoviruses (tobacco mosaic virus, TMV, common strain U1 and cruciferous TMV strain) containing amino-terminal hexahistidine affinity tags were overexpressed in Escherichia coli and purified by metal chelate affinity chromatography. Purified recombinant proteins were immobilized to a Ni(2+)-chelate adsorbent and their ability to interact with full-length genomic TMV RNA was tested. Here we report that binding of viral RNA to hexahistidine fusion movement proteins results in the formation of stable ribonucleoprotein complexes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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