| Autor: |
Balagopal P; Department of Medicine, Endocrinology and Metabolism, University of Vermont, Burlington 05405., Nair KS, Stirewalt WS |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1994 Aug 15; Vol. 221 (1), pp. 72-7. |
| DOI: |
10.1006/abio.1994.1381 |
| Abstrakt: |
A convenient procedure for the isolation of milligram quantities of myosin heavy chain (MHC) from small samples of muscle tissue using preparative gel electrophoresis is described. Application of the methodology to measurements of the fractional synthesis rates (ks) of MHC using skeletal muscle biopsy samples from humans and cardiac tissue from pigs following continuous intravenous infusions of stable isotope (L-[1-13C]-leucine) is demonstrated. The sensitivity and reproducibility of the determination of stable isotope enrichment are also defined. In addition, measurements of the ks of MHC in skeletal muscle of rats in vivo using radioisotope-tracer methodologies are described. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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