Phylogenetic analysis of segment 10 from African horsesickness virus and cognate genes from other orbiviruses.

Autor: de Sá RO; Department of Biology, University of Richmond, VA 23173., Zellner M, Grubman MJ
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 1994 Aug; Vol. 33 (2), pp. 157-65.
DOI: 10.1016/0168-1702(94)90052-3
Abstrakt: Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolutionary relationships among serotypes using cladistics. Based on this analysis the data support a close relationship between serotypes 4 and 9 and between serotypes 1 and 8 and a closer relationship of serotype 3 to the 4 and 9 group.
Databáze: MEDLINE