[Cloning cDNA of human S26 ribosomal protein and determination of its primary structure].
| Molecular Sequence: | GENBANK X77770 |
|---|---|
| Substance Nomenclature: | 0 (DNA, Complementary) 0 (RPS26 protein, human) 0 (Ribosomal Proteins) 0 (Rps26 protein, rat) |
| Entry Date(s): | Date Created: 19940601 Date Completed: 19941028 Latest Revision: 20061115 |
| Update Code: | 20221213 |
| PMID: | 7945460 |
| Autor: | Filipenko ML, Vladimirov SN, Muravlev AI, Karpova GG, Mertvetsov NP |
| Jazyk: | ruština |
| Zdroj: | Bioorganicheskaia khimiia [Bioorg Khim] 1994 Jun; Vol. 20 (6), pp. 644-9. |
| Abstrakt: | The polymerase chain reaction technique was employed to isolate cDNA encoding S26 human ribosomal protein. Based on the known sequence of rat S26 ribosomal protein we have designed primers and amplified the corresponding sequence of human cDNA from total placenta cDNA. The fragment of S26 cDNA was cloned in a plasmid vector and sequenced by the Sanger method. Extremely high homology (87.7%) between coding regions of S26 mRNAs in rat liver and human placenta was revealed. The only amino acid substitution (Ser-->Val) at position 38 was observed. Results of blot hybridization with partially digested human genomic DNA suggest the presence of more than 6 copies of the S26 gene. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|