| Autor: |
Luehrsen KR; Department of Biological Sciences, Stanford University, California 94305-5020., Walbot V |
| Jazyk: |
angličtina |
| Zdroj: |
Genes & development [Genes Dev] 1994 May 01; Vol. 8 (9), pp. 1117-30. |
| DOI: |
10.1101/gad.8.9.1117 |
| Abstrakt: |
Intron recognition in Angiosperms is hypothesized to require AU-rich motifs within introns. In this report we examined the role of AU-rich motifs in pre-mRNA processing. AU-rich segments of maize introns inserted near the single intron of the maize Bronze-2(Bz2) gene result in alternative splicing. Other insertions of AU-rich sequence in the Bz2 cDNA resulted in de novo intron creation using splice junctions at the edges of the AU-rich region. Surprisingly, the five AU-rich inserts that we tested also caused polyadenylation, even though none had been selected for that function in plants. Insertions of GC-rich sequence into Bz2 did not cause either splicing or polyadenylation. We propose that AU-rich motifs are a general signal for RNA processing in maize and that in the absence of a 5' splice site, polyadenylation is the default pathway. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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