Identification of a chaperonin-10 homologue in plant mitochondria.

Autor: Burt WJ; Department of Plant Sciences, University of Oxford, UK., Leaver CJ
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 1994 Feb 14; Vol. 339 (1-2), pp. 139-41.
DOI: 10.1016/0014-5793(94)80401-x
Abstrakt: Chaperonin-60 and chaperonin-10 form stable binary complexes in the presence of ATP. This phenomenon has been used as the basis for the identification of a chaperonin-10 homologue in potato (Solanum tuberosum L.) mitochondria. ATP-dependent binary complexes formed between chaperonin-60 and chaperonin-10 in potato mitochondrial extracts were isolated by affinity chromatography on a column of immobilised chaperonin-60 antibodies. Partial amino acid sequence analysis of the chaperonin-10 protein indicates that it is related in primary structure to rat mitochondrial chaperonin-10.
Databáze: MEDLINE