| Autor: |
Clingen PH; MRC Cell Mutation Unit, Sussex University, Falmer, Brighton, United Kingdom., Arlett CF, Cole J, Waugh AP, Lowe JE, Harcourt SA, Hermanova N, Roza L, Mori T, Nikaido O, et. al. |
| Jazyk: |
angličtina |
| Zdroj: |
Photochemistry and photobiology [Photochem Photobiol] 1995 Feb; Vol. 61 (2), pp. 163-70. |
| DOI: |
10.1111/j.1751-1097.1995.tb03955.x |
| Abstrakt: |
By using specific monoclonal antibodies in situ and a computer-assisted image analysis system we have determined the relative induction of cyclobutane dimers, (6-4) photoproducts and Dewar isomers in human mononuclear cells and fibroblasts following irradiation with UVC, broad-spectrum UVB and narrow-spectrum UVB. The lamps produced these lesions in different proportions, with broad-spectrum UVB inducing a greater combined yield of (6-4) photoproducts and Dewar isomers per cyclobutane dimer than UVC or narrow-spectrum UVB. The relative induction ratios of (6-4) photoproducts compared to cyclobutane dimers were 0.15, 0.21 and 0.10 following irradiation with UVC, broad- or narrow-spectrum UVB, respectively. Although Dewar isomers were induced by UVC, their relative rate of formation compared to cyclobutane dimers was significantly greater after irradiation with either broad-spectrum or narrow-spectrum UVB. These values were 0.001, 0.07 and 0.07, respectively. With each lamp source, we have determined the survival of normal human T-lymphocytes and fibroblasts at fluences, which induce equivalent yields of cyclobutane dimers, (6-4) photoproducts or (6-4) photoproducts plus Dewar isomers. Killing of fibroblasts appears to be associated with (6-4) photoproduct formation, whereas killing of T-lymphocytes seems to be mediated by combined (6-4) plus Dewar yields. These results emphasize the need to study the biological effects of UVB because cellular responses may be different from those following UVC irradiation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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