| Abstrakt: |
Levels of cyclic nucleotides (CN) in cardiac, cerebral, and hepatic tissues were measured in the early postresuscitation period in rats exposed to systemic circulation arrest induced by clamping of the cardiac vascular bundle. Changes of CN levels in the examined tissues were different in principle. In the heart cAMP levels progressively reduced 30 and 60 min after resuscitation, whereas cGMP levels were increasing. On the contrary, in the liver cAMP levels increased and cGMP content reliably reduced. Levels of cAMP and cGMP reliably reduced in the basis cerebri, with a trend to their reduction observed in the hemispheric cortex. Lysosomal hydrolases were activated in the said parts of the brain, their fraction was isolated in percoll gradient 30 min after resuscitation. In lysosomal fraction isolated from cardiac and hepatic tissues at the same period no activation of acid phosphatase and cathepsin D was observed in comparison with the control. It may be assumed that activation of lysosomal enzymes is governed by CN deficit. |
