| Autor: |
Gillespie KM; Department of Medicine, University of Cambridge, UK., Qasim FJ, Tibbatts LM, Thiru S, Oliveira DB, Mathieson PW |
| Jazyk: |
angličtina |
| Zdroj: |
Scandinavian journal of immunology [Scand J Immunol] 1995 Mar; Vol. 41 (3), pp. 268-72. |
| DOI: |
10.1111/j.1365-3083.1995.tb03563.x |
| Abstrakt: |
Mercuric chloride (HgCl2) induces autoimmunity in Brown Norway (BN) rats, with necrotizing vasculitis in the gut. Circumstantial evidence implicates the Th2 subset of CD4+ T lymphocytes, which produces IL-4. We developed a quantitative polymerase chain reaction (PCR) technique to quantify IL-4 gene expression. A phagemid containing rat IL-4 cDNA was modified to act as the template for a synthetic RNA construct; a known amount of synthetic RNA was added to total RNA from spleen and caecum of BN rats at various times after HgCl2, followed by reverse transcriptase PCR. IL-4 gene expression increased markedly in spleen and caecum after HgCl2. Splenic levels peaked by 10 days at approximately five-times baseline, then returned towards normal as the autoimmune response was spontaneously regulated. Caecal IL-4 expression peaked at 48 h, at which time we observed a previously unreported early phase of tissue injury, with necrotizing vasculitis qualitatively similar to that reported previously in the later phases of the model. These data support a key role for IL-4 in this experimental model of autoimmunity. The quantitative PCR technique can be modified for analysis of other cytokines, allowing further investigation of the role of T cell subsets in this model. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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