[Detection of Brucella with an automatic hemoculture system: Bact/Alert].

Autor:	Casas J; Servicio de Microbiología, Hospital Virgen de las Nieves, Granada., Partal Y, Llosá J, Leiva J, Navarro JM, de la Rosa M
Jazyk:	Spanish; Castilian
Zdroj:	Enfermedades infecciosas y microbiologia clinica [Enferm Infecc Microbiol Clin] 1994 Dec; Vol. 12 (10), pp. 497-500.
Abstrakt:	Background: The ability of in vitro and in vivo detection of Brucella spp. with the Bact/Alert system was studied. Methods: Three strains of Brucella melitensis and two of Brucella abortus were used. Different dilutions of the five strains were performed in trypticase soy broth (TSB), achieving concentrations of 1 cfu/ml, 5 cfu/ml, 10 cfu/ml and 100 cfu/ml. Ten ml of each dilution and strain were inoculated into 5 aerobic bottles Bact/Alert and 5 biphasic Hemóline bottles. Furthermore, over a 9 month period, 8,216 bottles of Bact/Alert bottles from hospitalized patients and from the emergency department were processed in the authors' laboratory. Results: The mean detection time for Brucella growth was from 2 to 3 days with the Bact/Alert system, and 14 days in the biphasic bottles. Former bottles processed in the authors' laboratory, 11 aerobic bottles belonged to 5 patients in whom brucelosis was confirmed by bloodculture. The Bact/Alert system detected Brucella melitensis in only on bottle at 2.9 days of incubation. In 7 bottles Bact/Alert detected B. melitensis by a blind pass of these bottles at 10 to 20 days of incubation. Conclusions: These results suggest that the Bact/Alert system does not totally solve the diagnosis of brucellosis. Blind passes of the bloodcultures are required.
Databáze:	MEDLINE
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