| Autor: |
Andersland JM; Southern Regional Research Center, New Orleans, Louisiana., Fisher DD, Wymer CL, Cyr RJ, Parthasarathy MV |
| Jazyk: |
angličtina |
| Zdroj: |
Cell motility and the cytoskeleton [Cell Motil Cytoskeleton] 1994; Vol. 29 (4), pp. 339-44. |
| DOI: |
10.1002/cm.970290406 |
| Abstrakt: |
Anti-actin monoclonal antibodies were prepared using phalloidin-stabilized actin that was purified from pea roots by DNase I affinity chromatography. One monoclonal antibody, designated mAb3H11, bound plant actin in preliminary screenings and was further analyzed. Immunoblot analysis showed that this antibody had a high affinity for plant actin in crude and purified preparations but a low affinity for rabbit muscle actin. In immunoblots of plant extracts separated on two-dimensional gels it appeared to bind all actin isoforms recognized by the JLA20 anti-chicken actin antibody. Using immunofluorescent cytochemistry, the antibody was used to observe actin filaments in aldehyde-fixed and methanol-treated tobacco protoplasts. These results indicate that mAb3H11 should be a useful reagent for the study of plant actins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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