| Autor: |
Cuneo A; Institute of Hematology, University of Ferrara, Italy., Bigoni R, Balboni M, Carli MG, Piva N, Fagioli F, Latorraca A, Wlodarska I, van den Berghe H, Castoldi G |
| Jazyk: |
angličtina |
| Zdroj: |
Leukemia & lymphoma [Leuk Lymphoma] 1994 Sep; Vol. 15 (1-2), pp. 167-72. |
| DOI: |
10.3109/10428199409051693 |
| Abstrakt: |
Fluorescent in situ hybridization (FISH) with a chromosome 12-specific pericentromeric probe was performed in 42 patients with B-cell chronic lymphocytic leukemia (CLL) and in 10 patients with hairy cell leukemia (HCL). In all cases, a normal karyotype in more than 10 metaphase cells was obtained by conventional chromosome study. FISH documented that 6/42 patients with CLL in fact had trisomy 12 in 15-49% interphase cells. Sequential FISH studies were performed in 2 cases, showing an increase of percentage of trisomic cells over a 2-month to 4-year period. Two out of 10 patients with HCL, one of whom had morphologic features consistent with a diagnosis of HCL variant, showed 5.5 and 10% interphase nuclei with three fluorescent signals, a finding suggestive of the presence of trisomy 12. Combined immunophenotyping and FISH staining in these patients with HCL documented that trisomic cells were CD11c-positive, CD13-negative, and CD2-negative. We conclude that FISH is a sensitive technique allowing for the detection of trisomy 12 in a fraction of cytogenetically normal patients affected with CLL and HCL. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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