Production and characterization of a monoclonal antibody specific for the E1 component of the pyruvate dehydrogenase complex.

Autor: Stafejeva OA; A.N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow., Katrukha AG, Bulargina TV, Khailova LS
Jazyk: angličtina
Zdroj: Biochemistry and molecular biology international [Biochem Mol Biol Int] 1994 Aug; Vol. 34 (1), pp. 9-15.
Abstrakt: The monoclonal antibody F7F10 against the E1 component of the pigeon breast muscle pyruvate dehydrogenase complex has been produced. The dissociation constant of the E1-mAb F7F10 complex was determined to be 5.93 x 10(-8)M. The cross-reaction of the mAb with the E1 components of the pyruvate dehydrogenase complexes from various species (including human) was established. The competitive solid-phase immunoenzyme assay of the E1 component and PDC concentrations has been developed.
Databáze: MEDLINE