| Autor: |
Rotbart HA; Department of Pediatrics, University of Colorado School of Medicine, Denver., Sawyer MH, Fast S, Lewinski C, Murphy N, Keyser EF, Spadoro J, Kao SY, Loeffelholz M |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of clinical microbiology [J Clin Microbiol] 1994 Oct; Vol. 32 (10), pp. 2590-2. |
| DOI: |
10.1128/jcm.32.10.2590-2592.1994 |
| Abstrakt: |
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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