| Autor: |
Bianchi AT; Department of Immunology, Central Veterinary Institute, Lelystad, Netherlands., Moonen-Leusen HW, van der Heijden PJ, Bokhout BA |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 1995 Feb; Vol. 44 (3-4), pp. 309-17. |
| DOI: |
10.1016/0165-2427(94)05307-e |
| Abstrakt: |
Double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) have been developed for the assessment of IgM, IgG and IgA concentrations in porcine serum. Isotype-specific monoclonal antibodies (mAb) were used for coating and detection. The DAS-ELISAs were examined for their ability to detect each isotype and for the assay variations. A computer programme was used to verify the parallelism of the slope of each serum sample with the slope of standard/reference serum, a prerequisite for reliable estimation of Ig concentrations. The DAS-ELISAs are easy to perform and highly specific, have adequate detection levels (ranging from 7 to 50 ng ml-1) and are very reproducible, as illustrated by the inter- and intra-assay variation coefficients (ranging between 4.9 and 7%). To illustrate the applicability of the ELISAs we assessed Ig concentrations in pig sera sampled from birth to young adulthood. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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