| Autor: |
Xu X; Department of Biochemistry, Tokyo Medical and Dental University School of Medicine., Yamamura Y, Tsukada T, Yoshida MA, Senda H, Nagayoshi M, Ikeuchi T, Ikawa Y |
| Jazyk: |
angličtina |
| Zdroj: |
Japanese journal of cancer research : Gann [Jpn J Cancer Res] 1995 Mar; Vol. 86 (3), pp. 284-91. |
| DOI: |
10.1111/j.1349-7006.1995.tb03052.x |
| Abstrakt: |
Two different erythroleukemia cell lines have been established from the splenic lesions of transgenic mice possessing the Friend spleen focus-forming virus (F-SFFV) gp55 gene. One showed a near-diploid karyotype and a temperature-sensitive (ts) p53 mutation, and the other, a hyper-triploid karyotype with double p53 mutations found by single-strand conformation polymorphism (SSCP) analysis. The cell lines both retained No.11 chromosomes on which p53 genes are localized. Another p53 allele in the cell line with the ts-p53 mutation appeared intact in the SSCP analysis of the genomic exon 5. The cells with the ts-mutant p53 gene showed no apparent change with temperature shift in their growth or dimethylsulfoxide-induced differentiation, although the wild-type p53 gene on the other allele was not expressing. This ts-p53Val-135 gene made p53-deficient fibroblasts anchorage-independent at 37 degrees C but not at 32 degrees C. This non-virus-producing, mouse erythroleukemia cell line will be useful for the study of mutated p53 function during the induction of erythrodifferentiation or apoptotic change. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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