| Autor: |
Breedveld MW; Department of Food Science, Pennsylvania State University, University Park 16802., Miller KJ |
| Jazyk: |
angličtina |
| Zdroj: |
Microbiology (Reading, England) [Microbiology (Reading)] 1995 Mar; Vol. 141 ( Pt 3), pp. 583-8. |
| DOI: |
10.1099/13500872-141-3-583 |
| Abstrakt: |
The transfer of phosphoglycerol moieties from phosphatidylglycerol to the cyclic (1,2)-beta-glucans in growing cultures of Rhizobium meliloti strain 1021 was investigated using pulse-chase experiments with [3H]glycerol and/or [14C]glucose. No transfer occurred when cells were grown and pulse-chased in a medium containing 0.4 M NaCl. However, radiolabelled glycerophosphorylated cyclic (1,2)-beta-glucans could be detected within 30 min after transfer of these cultures to a low-osmolarity medium. Conversely, when low-osmolarity cultures were shifted to a high-osmolarity medium containing 0.4 M NaCl or 0.8 M sucrose, the transfer of phosphoglycerol substituents to the cyclic (1,2)-beta-glucans was inhibited. Further experiments revealed that the transfer of phosphoglycerol substituents to the cyclic (1,2)-beta-glucans occurs within the periplasmic compartment. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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