| Autor: |
Rivard CH; Department of Orthopaedic Surgery, Hôpital Sainte-Justine, Montréal, Canada., Chaput CJ, DesRosiers EA, Yahia LH, Selmani A |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of applied biomaterials : an official journal of the Society for Biomaterials [J Appl Biomater] 1995 Spring; Vol. 6 (1), pp. 65-8. |
| DOI: |
10.1002/jab.770060109 |
| Abstrakt: |
A natural poly(hydroxybutyrate-co-9% hydroxyvalerate) copolyester was processed into a three-dimensional porous foam structure by salt leaching/solvent casting with previously sieved sodium chloride salts. Laboratory-built P(HB-9% HV) foams and commercial collagen sponges were cut into small rectangular specimens, sterilized, and prewetted using ethanol, rinsed with Dulbecco's minimum essential medium + 10% serum culture media, and seeded with fibroblasts isolated from canine anterior cruciate ligaments. The fibroblast cultures into such porous substrates were performed from 0 to 35 days by incubation (5% CO2) at 37 degrees C. It demonstrated that the P(HB-HV) sustained a cell proliferation rate similar to that observed in collagen sponges, up to at least 35 days, with a maximal cell density on the day 28 in culture. On the other hand, the P(HB-HV) materials kept their structural integrity during the culture period while the collagen foams contracted greatly. Further, the total protein production after 4 weeks in culture was found to be twice as high (190 +/- 10%) in the P(HB-9% HV) foam than in the collagen foam. Porous P(HB-HV) materials appear to be adequate polymeric substrates for cell cultures. However, further evaluations are still required to confirm such preliminary results. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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