| Abstrakt: |
The component composition of the pentaene antibiotic roseofungin was studied by the phase reversal high performance liquid chromatography in the Millichrom apparatus. A 62 x 2 mm column filled with the Separon 5 C18 sorbent was employed. The composition of the mobile phase was determined experimentally by testing various solvent mixtures (methanol-water, methanol-acetate buffer, 0.05 M, pH 4.7, acetonitrile acetate buffer) with different contents of the organic solvent under conditions of isocratic and gradient elution. The optimal separation of the complex was achieved with the use of the mixture of acetonitrile and acetate buffer (45:55) as the mobile phase. Roseofungin was detected at 360 nm. The assay of the roseofungin experimental lots showed that roseofungin mainly contained three components with identical UV spectra. Their contents ranged within 8-17, 45-57 and 25-30 per cent respectively. Some of the samples additionally contained minor components whose quantity did not exceed 20 per cent. |
