| Autor: |
Fleming TE; Department of Dermatology, Case Western Reserve University, Cleveland, Ohio., Mirando WS, Trefzer U, Tubesing KA, Elmets CA |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of investigative dermatology [J Invest Dermatol] 1993 Nov; Vol. 101 (5), pp. 754-8. |
| DOI: |
10.1111/1523-1747.ep12371688 |
| Abstrakt: |
The B7 adhesion molecule, a member of the immunoglobulin superfamily, has previously been identified primarily on cells of hematopoietic origin. Because B7 has been shown to facilitate interactions with T cells and because cells of the epidermis are proficient at binding and activating T lymphocytes, studies were performed to determine whether B7 was expressed in human epidermis. A subpopulation of brightly staining B7-positive cells was observed in situ in normal human epidermis. Flow-cytometric examination of epidermal cell suspensions that had been cultured for 24 h or longer demonstrated that between 10 and 40% of cells expressed B7 or a closely related antigen. Immunoelectron microscopy, double-staining procedures, and examination of epidermal suspensions depleted of Langerhans cells all confirmed that the B7-positive cells were keratinocytes. These studies identify human epidermal keratinocytes, a non-hematopoietic cell population, as a cell type capable of expressing a B7-like adhesion molecule. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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