| Autor: |
Wagner RW; Gilead Sciences, Inc., Foster City, CA 94404., Matteucci MD, Lewis JG, Gutierrez AJ, Moulds C, Froehler BC |
| Jazyk: |
angličtina |
| Zdroj: |
Science (New York, N.Y.) [Science] 1993 Jun 04; Vol. 260 (5113), pp. 1510-3. |
| DOI: |
10.1126/science.7684856 |
| Abstrakt: |
Phosphorothioate oligodeoxynucleotides containing the C-5 propyne analogs of uridine and cytidine bind RNA with high affinity and are potent antisense inhibitors of gene expression. In a cellular assay, gene-specific antisense inhibition occurred at nanomolar concentrations of oligonucleotide, was dose-dependent and exquisitely sensitive to sequence mismatches, and was correlated with the melting temperature and length of oligonucleotide. Activity was independent of RNA target site and cell type but was detectable only when the oligonucleotides were microinjected or delivered with cell-permeabilizing agents. These oligonucleotides may have important applications in therapy and in studies of gene function. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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