| Autor: |
Addison WR; Department of Genetics, University of Alberta, Edmonton, Canada., Brook WJ, Querengesser LD, Tiong SY, Russell MA |
| Jazyk: |
angličtina |
| Zdroj: |
Genome [Genome] 1995 Aug; Vol. 38 (4), pp. 724-36. |
| DOI: |
10.1139/g95-091 |
| Abstrakt: |
In Drosophila, imaginal discs are the undifferentiated larval precursors of the pattern of epidermal and sensory neural cells in each adult segment. Although cell fates are already specified by late third instar, disc fragments can either regenerate or duplicate after growth in culture. The outcome depends on signaling between cells across the healed wound and involves a redeployment of the expression patterns of selector genes and other disc pattern genes. We recently used the enhancer-trap method to screen for such genes that are expressed ectopically at the wound-heal site in imaginal discs undergoing regeneration. Here we report the cloning by plasmid rescue of transcribed sequences adjacent to one such enhancer-trap insertion. Using Northern analysis and in situ hybridization we show that one transcript is expressed in the embryo and in imaginal discs in a pattern similar to that of the enhancer trap. We also, by imprecise excision of the enhancer-trap insertion, generated a series of flanking deletions that were mapped using Southern analysis and complementation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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