| Autor: |
Bundgaard JR; Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark., Hansen TO, Friis-Hansen L, Rourke IJ, van Solinge WW, Nielsen FC, Rehfeld JF |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1995 Aug 07; Vol. 369 (2-3), pp. 225-8. |
| DOI: |
10.1016/0014-5793(95)00754-w |
| Abstrakt: |
Studies of transgenic mice have shown that transcriptional control of the gastrin gene exhibits significant species differences. Transfection of the human gastrin promoter in murine cells have depicted proximal Sp1, E-box and CACC elements as the major determinants of transcription. We have examined cis-regulatory elements of the human promoter on a human gastrin expressing cell line and find that a distal -135 to -142 Sp1 element is necessary for maximal activity. Alignment of the mouse and human promoters shows that the proximal human Sp1 and CACC elements are not conserved, whereas the E-box element is retained. The distal Sp1 element is present in mouse but exhibits a C to T transition in the core that is likely to reduce binding affinity of Sp1. We conclude that gastrin gene transcription is regulated by distinct elements in man and rodents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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