| Autor: |
Stromskaya TP; Institute of Cancerogenesis, Cancer Research Center, Moscow, Russian Federation., Grigorian IA, Ossovskaya VS, Rybalkina EY, Chumakov PM, Kopnin BP |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1995 Jul 17; Vol. 368 (2), pp. 373-6. |
| DOI: |
10.1016/0014-5793(95)00662-s |
| Abstrakt: |
We compared the influence of exogenous N-ras oncogene and treatment with PKC agonist 12-O-tetradecanoylphorbol-13-acetate (TPA) on P-glycoprotein (Pgp) function in various human, rat and dog cell lines. Two approaches were used: (a) flow cytometry analysis of Rhodamine 123 (Rh123) exclusion; and (b) sensitivity to cytotoxic action of colchicine. We have found that in Rat1 fibroblasts, rat IAR2 epithelial cells and rat McA RH 7777 (hepatoma), ras activates Pgp function, while in MDCK (dog kidney), K562 (human chronic myelogenous leukaemia) and LIM1215 (human colon carcinoma) cells it either has no effect or even acts in opposite direction. TPA-induced Pgp function shows dissimilar pattern of cell specificity. It is assumed that PKC and ras oncogene regulate mdr1 gene expression through at least partially distinct signalling pathways. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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