| Autor: |
Fuqua JS; Division of Pediatric Endocrinology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA., Sher ES, Migeon CJ, Berkovitz GD |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical chemistry [Clin Chem] 1995 Aug; Vol. 41 (8 Pt 1), pp. 1146-9. |
| Abstrakt: |
Determination of the plasma concentration of testosterone (T) is important in evaluating infants born with ambiguous genitalia and micropenis, and several commercially available kits provide a direct assay of T in unextracted plasma. Using plasma samples obtained from 36 subjects < 6 months old, we compared the concentration of plasma T measured by RIA after extraction and purification by column chromatography with the T concentration measured in a direct assay. When aliquots of samples were purified before RIA, the concentration of T was markedly lower than in the direct assay. In the first 3 weeks postpartum, results of the direct assay were 3.8-fold greater than those obtained after purification. This difference decreased over time, and by age 2 months there was fairly good agreement between the two methods. These data indicate that some direct assays of plasma T are inappropriate during the first 2 months postpartum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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