| Autor: |
Shchyolkina AK; V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow., Borisova OF, Minyat EE, Timofeev EN, Il'icheva IA, Khomyakova EB, Florentiev VL |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1995 Jun 19; Vol. 367 (1), pp. 81-4. |
| DOI: |
10.1016/0014-5793(95)00519-f |
| Abstrakt: |
Oligonucleotides 5'-d(CT)5-L-d(AG)5-L-d(GA)5-3' and 5'-d(GA)5-L-d(TC)5-L-d(GA)5-3' [L = pO(CH2CH2O)3p] were studied by thermal denaturation, chemical modification and binding of fluorescent dyes. Both oligonucleotides are shown to fold back on itself twice forming at pH 7 a sufficiently stable triplex ether with antiparallel-oriented oligopurine strands (the first compound) or parallel-oriented oligopurine strands (the second compounds). The parallel triplex is significantly less stable than the antiparallel one. On the basis of conformational modeling, possible types of base tripling in the triplets are proposed. Thus our data provide the first convincingly evidence for the existence of a purine-pyrimidine-purine triplex with parallel orientation of identical strands. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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