| Autor: |
el-Rahman A; Department of Biochemistry, Faculty of Medicine, Ain Shams University, Cairo, Egypt., Hammouda MA, Fakeir A |
| Jazyk: |
angličtina |
| Zdroj: |
Cytometry [Cytometry] 1995 May 01; Vol. 20 (1), pp. 19-22. |
| DOI: |
10.1002/cyto.990200105 |
| Abstrakt: |
Erythrocyte response to oxidant challenge has been used as an indicator of cell membrane tolerance to oxidant stress. This study describes a simple technique for evaluation of erythrocyte response to hydrogen peroxide oxidant stress using flow cytometry as an assay method. Challenged cells showed a detectable increase in fluorescence over the basal level. This increase in fluorescence was both concentration and time dependent. The test was standardized using 0.1 mM hydrogen peroxide and 2 h incubation. The standardized test showed a higher fluorescence of challenged umbilical cord erythrocytes compared to challenged adult red blood cells. We recommend further use of this test for examining other populations of red blood cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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