| Abstrakt: |
The luminescent photoprotein aequorin was used to measure intracellular free Ca2+ in three species of suctorian protozoon, Trichophrya riederi, Trichophrya collini and Trichophrya rotunda. Resting [Ca2+]i ranged from about 75-110 nM, and was unaffected by a change in temperature of the perfusate. Spontaneous Ca2+ transients were observed in all three species, with peak amplitudes ranging from 100-600 nM. In T. riederi and T. rotunda, three categories of transient (small, intermediate, large) were recorded; T. collini displayed only small transients. In both T. riederi and T. collini, raising the temperature from 5 degrees to 26 degrees C led to an increase in the frequency of transients. Furthermore, in T. riederi, large transients occurred only at the higher temperature. The frequency of spontaneous contractions of the tentacles of T. riederi was also temperature-dependent. Increasing the temperature over the range 5-26 degrees C led to a concomitant increase in contraction frequency and a decrease in mean tentacle length. The possible mechanisms of spontaneous Ca2+ transient generation and their role in the control of contraction are discussed. |
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