| Autor: |
Hung GL; Ferguson Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, PA 15261, USA., Galea-Lauri J, Mueller GM, Georgescu HI, Larkin LA, Suchanek MK, Tindal MH, Robbins PD, Evans CH |
| Jazyk: |
angličtina |
| Zdroj: |
Gene therapy [Gene Ther] 1994 Jan; Vol. 1 (1), pp. 64-9. |
| Abstrakt: |
We have developed an ex vivo method for delivering genes to the synovial lining of joints and expressing them intra-articularly. The present studies were designed to determine whether transfer of a human interleukin-1 receptor antagonist protein (IRAP) gene by this method was able to antagonize the intra-articular actions of interleukin-1. Intra-articular injections of human recombinant interleukin-1 beta (hrIL-1 beta) into the knees of control rabbits provoked a marked leukocytic infiltrate into the joint space, severe synovial thickening and hypercellularity, and loss of proteoglycans from articular cartilage. Genetically modified knees contained several nanograms of human IRAP and inhibited each of these effects of IL-1 beta. These data demonstrate for the first time that delivery of an appropriate gene to joints can prevent intra-articular pathology. Such findings permit cautious optimism about the eventual development of a gene treatment for arthritis and other disorders of the joint. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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