| Autor: |
Szekeres G; Laboratory of Histopathology, IMMUNOTECH, Marseille, France., Le Tourneau A, Benfares J, Audouin J, Diebold J |
| Jazyk: |
angličtina |
| Zdroj: |
Pathology, research and practice [Pathol Res Pract] 1995 Feb; Vol. 191 (1), pp. 52-6. |
| DOI: |
10.1016/S0344-0338(11)80923-4 |
| Abstrakt: |
Immunostaining of the cell cycle-associated Ki-67 antigen was studied on routinely formalin-fixed and paraffin-embedded tissue sections, using the Ki-67-specific monoclonal antibody MIB-1. Immunomorphological analysis of the Ki-67 immunostaining pattern was carried out following tissue pre-treatments including combinations of microwave heating and trypsinization, as well as of ribonuclease and deoxyribonuclease pre-digestion of the sections. The nucleolar Ki-67 immunostaining after slide pre-treatment by microwave heating followed by trypsinization was reduced only by ribonuclease pre-digestion, if this latter was used before heating. Ki-67 immunostaining was not significantly reduced by deoxyribonuclease treatment. We conclude that Ki-67, located in the nucleoli, may be associated there with nucleolar RNA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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