| Autor: |
Jakob C; Max-Planck-Institut für experimentelle Medizin, Abt. Immunchemie, Göttingen, Germany., Götz H, Hellmann T, Hellmann KP, Reymann S, Flörke H, Thinnes FP, Hilschmann N |
| Jazyk: |
angličtina |
| Zdroj: |
FEBS letters [FEBS Lett] 1995 Jul 10; Vol. 368 (1), pp. 5-9. |
| DOI: |
10.1016/0014-5793(95)00465-l |
| Abstrakt: |
In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC. Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues. Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells. Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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