| Autor: |
Kelly WG, de Leon O, Rizkallah TH |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of clinical endocrinology and metabolism [J Clin Endocrinol Metab] 1978 Mar; Vol. 46 (3), pp. 445-51. |
| DOI: |
10.1210/jcem-46-3-445 |
| Abstrakt: |
A mixture of [19-3H]hydroxyandrostenedione and [14C]androstenedione was administered intravenously to 3 women and urine was collected. Only negligible radioactivity could be extracted from the untreated urine. Most of the 14C but only 11% of the 3H was rendered solube in organic solvents by beta-glucuronidase. [3H-19]hydroxyandrostenedione was recovered from this fraction. The conjugates remaining in the urine were extracted into CHCl3 as their pyridinium salts. After solvolysis of the extract with HCLO4 in tetrahydrofuran, neutral metabolites were obtained. Substances extractable from water with organic solvents were obtained by solvolysis of the conjugates with perchloric acid in tetrahydrofuran. [3H-19]hydroxyandrostenedione was identified by isotopic dilution as the major product of solvolysis. Thus, 19-hydroxyandrostenedione undergoes conjugation with glucuronic acid and probably sulfuric acid, most likely at C-19. The major urinary metabolite is the sulfate-like conjugate. Reduction in ring A is less important than for other steroids. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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