| Autor: |
Sako D; Genetics Institute, Small Molecule Drug Discovery Group, Cambridge, Massachusetts 02140., Chang XJ, Barone KM, Vachino G, White HM, Shaw G, Veldman GM, Bean KM, Ahern TJ, Furie B, et. al. |
| Jazyk: |
angličtina |
| Zdroj: |
Cell [Cell] 1993 Dec 17; Vol. 75 (6), pp. 1179-86. |
| DOI: |
10.1016/0092-8674(93)90327-m |
| Abstrakt: |
The initial adhesive interactions between circulating leukocytes and endothelia are mediated, in part, by P-selectin. We now report the expression cloning of a functional ligand for P-selectin from an HL-60 cDNA library. The predicted amino acid sequence reveals a novel mucin-like transmembrane protein. Significant binding of transfected COS cells to P-selectin requires coexpression of both the protein ligand and a fucosyltransferase. This binding is calcium dependent and can be inhibited by a neutralizing monoclonal antibody to P-selectin. Cotransfected COS cells express the ligand as a homodimer of 220 kd. A soluble ligand construct, when coexpressed with fucosyltransferase in COS cells, also mediates P-selectin binding and is immunocrossreactive with the major HL-60 glycoprotein that specifically binds P-selectin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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