| Autor: |
Pisani T; Research Department, Genzyme Diagnostics, Cambridge, Massachusetts 02139, USA., Gebski CP, Leary ET, Warnick GR, Ollington JF |
| Jazyk: |
angličtina |
| Zdroj: |
Archives of pathology & laboratory medicine [Arch Pathol Lab Med] 1995 Dec; Vol. 119 (12), pp. 1127-35. |
| Abstrakt: |
Clinical laboratories currently estimate low-density lipoprotein cholesterol using the Friedewald formula, which requires fasting specimens and is subject to error with increasing triglyceride levels. We describe a rapid method for isolating low-density lipoproteins using the Direct LDL Immunoseparation Reagent for subsequent measurement of cholesterol by conventional assay. This method meets current guidelines for precision with within-run and run-to-run coefficients of variation of less than 3%. Results are in good agreement with the beta quantification reference method (Direct LDL-C = 1.03 [beta quantification] -0.06 mmol/L, [2.4 mg/dL] r = 0.980), there is minimal bias associated with increasing triglycerides or high-density lipoprotein cholesterol, and patient fasting is not required for accurate analysis. The Direct LDL Immunoseparation Reagent overcomes drawbacks of the Friedewald formula and appears to be suitable for accurate quantitation of low-density lipoprotein cholesterol in the routine laboratory. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
