| Autor: |
Tsuchiyama L; Bayer Corp., Berkeley, CA 94710, USA., Wong T, Kieran J, Boyle P, Penza D, Wetzel GD |
| Jazyk: |
angličtina |
| Zdroj: |
Human antibodies and hybridomas [Hum Antibodies Hybridomas] 1995; Vol. 6 (2), pp. 73-6. |
| Abstrakt: |
To examine the ability of normal and autoimmune individuals to produce circulating anti-TNF alpha antibodies, plasma samples from 10 RA patients, 10 SLE patients and 5 normal subjects were assessed for anti-TNF alpha antibody. While every individual tested demonstrated circulating IgM anti-TNF alpha antibody, IgG anti-TNF alpha autoantibody was seen predominantly in autoimmune patients. Only 1 of 5 normal individuals, but 15 of 20 autoimmune individuals had plasma IgG anti-TNF alpha antibodies. To examine the ability of normal and autoimmune individuals to produce anti-TNF alpha autoantibody from their circulating lymphocytes, EBV transformation was performed. Oligoclonal immortal cell lines were successfully established from 13 patients and each one secreted detectable IgM anti-TNF alpha autoantibody. Transformed cells from only 1 of 5 normal individuals secreted IgM anti-TNF alpha autoantibody. These results indicate a higher prevalence of anti-TNF alpha autoantibody production among autoimmune individuals although normal individuals are also capable of producing these autoantibodies. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
