Enhanced tumor cell expression of tumor necrosis factor receptors in head and neck squamous cell carcinoma.

Autor: von Biberstein SE; Department of Surgery, University of Connecticut Health Center, Farmington, USA., Spiro JD, Lindquist R, Kreutzer DL
Jazyk: angličtina
Zdroj: American journal of surgery [Am J Surg] 1995 Nov; Vol. 170 (5), pp. 416-22.
DOI: 10.1016/s0002-9610(99)80320-1
Abstrakt: Background: To determine if tumor necrosis factor (TNF) receptors are upregulated in tumor cells, we measured the distribution and levels of TNF-alpha and TNF-beta, and TNF receptors RI and RII, in head and neck squamous cell carcinoma (HNSCC) tumor specimens and normal control specimens.
Methods: HNSCC and control tissue specimens were analyzed qualitatively using immunohistochemistry and quantitatively using immunoassays.
Results: Immunohistochemical analysis revealed that TNF-alpha, TNF-beta, TNF RI, and TNF RII antigens were associated predominately with tumor cells in the tissue. Quantitative analysis of TNF factors and receptors in tissue homogenates (mean levels +/- standard error of the mean, in pg/mg of total protein) indicated that: (1) TNF-alpha levels in cancer patients were not statistically different from levels in normal tissues (7.27 +/- 0.91 versus 4.62 +/- 1.33, respectively, P < 0.11); (2) TNF-beta levels in cancer patients were one third of those in normal tissue (5.07 +/- 1.83 versus 16.06 +/- 3.26, respectively, P < 0.01); and (3) both TNF RI and TNF RII levels were consistently elevated two- to four-fold in the cancer tissue when compared to normal tissue levels (1,228.72 +/- 125.67 versus 650.33 +/- 187.70, P < 0.01; and 823.39 +/- 95.90 versus 230.03 +/- 153.01, P < 0.002, respectively).
Conclusions: In HNSCC, enhanced expression of TNF receptors on the cancer cells occurs and is likely to contribute to the regulation of TNF and its activation of tumor cells within the tumor microenvironment; targeting these receptors in cancer cells may provide a new approach to controlling tumor growth and metastasis.
Databáze: MEDLINE
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