| Abstrakt: |
Prostaglandin F2 alpha (PGF2 alpha) and its synthetic analog, 15-methyl-PGF2 alpha effects on the receptor content of estrogens and progesterone in the human decidual tissue were studied in vitro and in vivo experiments. The receptor content in vivo experiments was determined by Sketchard's technique and in vitro by the saturation method. Three hours after intravaginal administration of 15-methyl-PGF2 alpha suppositories to pregnant women the level of estrogen and progesterone receptors in decidual tissue cytosol significantly increased. Affinity of receptors with hormones remained unchanged. 10, 20, 50 and 100 mcg/ml concentrations of PGF2 alpha produced a significant and dose-dependent increase in the receptor content of estrogens following 15, 30, 60 and 180 minutes incubation of decidual tissue sections. The receptor level of progesterone remained unchanged. In experiments with inhibitors of protein and RNA biosynthesis, it was shown that an augmentation of the estrogen receptor content under PGF2 alpha action is not associated with biosynthesis of receptors de novo. |
