| Autor: |
Shideler CE, Stewart KK, Crump J, Wills MR, Savory J, Renoe BW |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical chemistry [Clin Chem] 1980 Sep; Vol. 26 (10), pp. 1454-8. |
| Abstrakt: |
We have examined the feasibility of the automated multiple flow-injection technique for application to clinical chemistry by adapting to this system the biuret method for the determination of total protein. Samples were discretely and rapidly introduced into a continuously flowing, nonsegmented reagent stream by means of an automatic sampler and high-pressure injection valve. Pumps operating at 1380-2070 kPa (200-300 psi) were utilized to introduce the biuret reagent and saline diluent into the system separately at flow rates of 72 and 47 microL/s, respectively. Use of 20-microL sample and a 3.0-s reaction-delay coil was adequately sensitive for analysis for total protein by this method. Samples were analyzed at a rate of 150/h with no detectable between-sample carryover. Within-run precision studies yielded relative standard deviations of 2.5% and less. Total protein values obtained by this method correlated well with those obtained by centrifugal analyzer and bubble-segmented continuous-flow biuret methods. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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