| Autor: |
Burstyn DG, McNicol LA, Voll MJ |
| Jazyk: |
angličtina |
| Zdroj: |
Infection and immunity [Infect Immun] 1980 Mar; Vol. 27 (3), pp. 889-96. |
| DOI: |
10.1128/iai.27.3.889-896.1980 |
| Abstrakt: |
As a first step toward developing a system of genetic exchange between Vibrio parahaemolyticus strains, spontaneously arising auxotrophic and Kanagawa phenomenon-negative (KP-) mutants were isolated and characterized. Auxotrophic mutants were selected by nalidixic acid enrichment of parental cultures. Some Cys- and Arg- mutants of a KP+ strain were found to be KP-. Reversion to prototrophy by these strains was not accompanied by a return to the parental KP+ phenotype. Additionally, two prototrophic KP- mutants were isolated. No detectable levels of vibriolysin were found in supernatant extracts of KP- mutants by slide gel immunodiffusion analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, or assay for lethal activity in mice. All Cys-, Arg-, and Pur- mutants tested reverted to a different auxotrophy (phenotypic interconversion) as well as to prototrophy. The possible role of insertion sequence-like elements in vibriolysin production and phenotypic interconversion is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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