| Autor: |
Scheres JM, Hustinx TW, Merkx GF |
| Jazyk: |
angličtina |
| Zdroj: |
Human genetics [Hum Genet] 1980 Feb; Vol. 53 (2), pp. 255-9. |
| DOI: |
10.1007/BF00273507 |
| Abstrakt: |
The morphologic changes occurring in human chromosomes during R-banding by Ba(OH)2 treatment were followed with the aid of bright-field and Nomarski interference contrast microscopy. It was found that the hot Ba(OH)2 pretreatment alone, i.e., without staining, caused a pattern of transverse ridges in the chromosomes that clearly corresponded to positive R-band regions. No chromosomal collapse could be seen during any stage of the R-banding procedure. Thus these events contrast with those observed in G-band formation with trypsin, where complete chromosomal collapse occurs after pretreatment and where staining is necessary to induce G-band ridges. The possible mechanism of R-band induction by Ba(OH)2 is discussed. It is proposed that the R-band ridges arise as a result of chromatin loss from the interband regions during the hot alkaline pretreatment. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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