| Autor: |
Monbaliu JG, Rosseel MT, Bogaert MG |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical sciences [J Pharm Sci] 1981 Aug; Vol. 70 (8), pp. 965-6. |
| DOI: |
10.1002/jps.2600700840 |
| Abstrakt: |
A high-performance liquid chromatographic method for the determination of methoxsalen in plasma was developed. The method includes extraction from plasma of the drug and the internal standard (5-methoxypsoralen) into methylene chloride. Chromatography was performed on a reversed-phase C8 column connected with a UV detector set at 254 nm. The mobile phase was methanol-acetonitrile-water (2:30:68). For a plasma sample of 0.25 ml, the maximal sensitivity was approximately 10 ng/ml. Accuracy was within 7.5% for therapeutic plasma levels, and the coefficient of variation varied between 4.3 and 0.9% for 28 and 300 ng/ml of plasma, respectively. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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