| Autor: |
Hosaka K, Kikuchi T, Mitsuhida N, Kawaguchi A |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of biochemistry [J Biochem] 1981 Jun; Vol. 89 (6), pp. 1799-803. |
| DOI: |
10.1093/oxfordjournals.jbchem.a133380 |
| Abstrakt: |
A rapid and sensitive spectrophotometric assay for free fatty acids using acyl-CoA synthetase and acyl-CoA oxidase is described. It is sensitive to as low as 5 nmol of free fatty acids, and the standard curve is linear up to 100 nmol. The assay consists of the measurement of H2O2 produced from free fatty acids by acyl-CoA synthetase and acyl-CoA oxidase. The quantity of H2O2 is determined by the absorbance at 550 nm in the presence of catalase and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT). This method shows a broad specificity to long-chain fatty acids and the recoveries of added fatty acids (C12-C18) are more than 90%. The presence or absence of serum components or Escherichia coli cell-free extracts has no significant effect on the recovery of added palmitic acid. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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