| Autor: |
Schroeder HR, Hines CM, Osborn DD, Moore RP, Hurtle RL, Wogoman FF, Rogers RW, Vogelhut PO |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical chemistry [Clin Chem] 1981 Aug; Vol. 27 (8), pp. 1378-84. |
| Abstrakt: |
A novel "sandwich" immunoassay for hepatitis B surface antigen monitored by chemiluminescence is described. The method involves use of an antibody-coated microtitration plate and requires 100 micro L of test specimen. The antigen binds to the antibody during the first 2-h incubation and, after an intermediate wash step, the sandwich is completed by 2-h incubation with antibody to antigen that has been labeled with an isoluminol derivative. A final wash step follows. A luminometer, built in-house, adds "microperoxidase" and peroxide, to initiate chemiluminescence, and provides automated readout at 10-s intervals. Results compare well in specificity and sensitivity with those of a comparison radioimmunoassay procedure. Within- and between-assay variability (CV) is 7 to 13% (n = 6). All reagents are stable at 4 degrees C for at least several months. Use of a non-radioisotopic label in this assay avoids the stability problems and inconvenience associated with radioactivity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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