Interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro.

Autor: Delinassios JG, Kottaridis SD
Jazyk: angličtina
Zdroj: Experimental cell biology [Exp Cell Biol] 1981; Vol. 49 (4), pp. 177-84.
DOI: 10.1159/000163821
Abstrakt: Lymphoblastoid (LB) cells interact in vitro with human fibroblasts. Cell complexes can be dissociated by trypsin treatment. Binding on fibroblasts is higher for Epstein-Barr virus-producer LB cells than for nonproducers, and depends on the origin of the fibroblastic cells. Stromal fibroblasts isolated from a metastatic lymph node tumor exhibited a highly increased potency of binding LB cells in comparison with fibroblasts isolated from normal breast epidermis or the primary breast tumor from the same patient. After a 10-hour interaction period, LB cells undergo abnormal divisions giving rise to the formation of 'mini cells'.
Databáze: MEDLINE