[Simple method for DNA fraction enrichment with unique genes].

Autor: Arsenian SG
Jazyk: ruština
Zdroj: Biokhimiia (Moscow, Russia) [Biokhimiia] 1980 Sep; Vol. 45 (9), pp. 1710-4.
Abstrakt: The conditions for RNA--DNA molecular hybridization in 15% phenolic suspension, allowing to extend the temperature range of the R-loop formation and render the method insensitive to the nucleotide composition of RNA are proposed. The method described results in a 100-fold enrichment of the globin-gene content in a few days. The method was used for enrichment of rat DNA preparations with globin genes.
Databáze: MEDLINE